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harmonize_intensities

Source: R/batch_correction.R
harmonize_intensities.Rd

Harmonize the expression values for a defined batch variable (batch_var).

Usage

harmonize_intensities(fcd, batch_var, seed = 91)

Arguments

fcd

flow cytometry dataset.

batch_var

vector of column names from fcd$anno$cell_anno to use for correcting the data.

seed

A seed is set for reproducibility.

Value

The function returns a fcd with a harmonized expression data frame based on HarmonyMatrix. The harmonized expression data frame is saved in fcd$expr$norm.

Details

harmonize_intensities

See [Korunsky et al., 2019](https://doi.org/10.1038/s41592-019-0619-0) for more details on the Harmony algorithm.